The Boztug Lab seeks to fill the open full-time position of a
TECHNICAL ASSISTANT in the field of GENETICS
The focus of the group is on the identification of rare monogenic disorders of the immune system and hematopoiesis, as well as inherited predisposition to cancer, to explain pathophysiological manifestations in humans (Salzer E et al Sci Immunol. 2020; Ghosh S et al Blood 2020; Serwas NK et al Nat Commun. 2019; Dominguez Conde et al J Clin Invest 2019; Ozen A et al NEJM 2017). In the context of this work, the Boztug group applies a combination of state-of-the-art genetic and genomic technologies including next generation sequencing (NGS) and a wide range of cellular and molecular techniques to elucidate novel pathways important for immune system homeostasis and predisposition to cancer in children.
The group works in a fully equipped molecular biology laboratory in a shared environment with cell culture, multi-color flow cytometry as well as all equipment needed for state-of-the-art NGS-based and genomic investigations such as whole exome sequencing, single-cell RNA sequencing, ATAC-Seq and others.
We are looking for a highly motivated and well-organized Technical Assistant, who will be fully integrated into the genetics core of the institute with an emphasis on genomic technologies leading to identification of disease-causing mutations. The new team member will contribute to the computer-based interpretation and post-variant-calling analysis of NGS-based genetic data, the overall operation of the lab, and the maintenance and curation of databases and research documentation. Thus, the position offers the great opportunity for career advancement by developing skills and knowledge in cutting-edge genetics, immunity and pediatric oncology.
Tasks and responsibilities include:
- Analysis and interpretation of NGS data to identify the relevant genetic variants underlying the patients’ conditions (incl. filtering and prioritization of potentially deleterious variants, genotype-phenotype correlations and validation and segregation of variants within family pedigrees)
- Handling disease cohorts and investigating the latest genetics technologies, databases, pipelines, etc.
- Documentation and reporting of genetic findings to external collaborators
- Maintenance and curation of a local database including information related to patients
- Genetics/Genomics: PCR (incl. primer design), conventional (Sanger) sequencing, next generation sequencing-based methods (e.g., exome sequencing, genome sequencing, ATAC-Seq, RNAseq)
- Contribution to general lab management as part of the weekly, bi-weekly, or monthly rotating lab duties schedules (e.g., ordering, preparation of general reagents, sample processing, and others) as well as training of new lab members
- Isolation and culturing of peripheral blood mononuclear cells (PBMCs) and nucleic acid isolation from human samples
- Molecular biology techniques (cloning, functional assays)
Requirements and desired qualifications:
- University or University of Applied Sciences Bachelor degree in Life Sciences; Master/PhD degree is considered a plus
- Proven experience in genetics, immunology and molecular biology techniques (e.g., DNA/RNA extraction, PCR); previous experience in NGS-based methods is advantageous
- Knowledge in public genetic databases (GnomAD, OMIM, Ensembl, Uniprot…) is advantageous
- Excellent written and oral communication skills in German (C1 or plus) and English
- High accuracy and reliability
- Excellent organizational and interpersonal skills, and perfect time management
- Ability to work in an international environment
- A challenging position in a meaningful, inspiring, international setting
- An outstanding working atmosphere in a strong team with excellent research and development opportunities
- The possibility of expanding your genetics/immunology knowledge with the most advanced techniques
- The option to set up and advance a quality management system partially independent and together with scientists from other research groups
- An excellent work climate and the possibility to join our social (cultural or sports) activities
- Flexible working hours, discounted lunch in our canteen and other great benefits
- Great location in the center of Vienna, a capital of biomedical research in Europe with excellent quality of life
- A fair and attractive salary package starting at € 2.500,-- gross (14x per year) on full-time basis with a view to increase based on your qualification and experience
The St. Anna Children’s Cancer Research Institute (CCRI), located in the center of Vienna, the world’s most livable city and one of Europe’s most important places for biomedical research and life sciences, is an international and multidisciplinary competence center striving to improve treatment of children and adolescents with cancer by connecting translational and clinical research with open-minded exploration of basic disease mechanisms. Through close cooperation between clinic and research, the CCRI provides an ideal environment for cutting-edge research and its translation into clinical practice. To achieve our ultimate goal of advancing the well-being of patients, the CCRI constantly pushes scientific boundaries and strongly promotes close collaboration and exchange with external institutions like the Medical University of Vienna, CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, the Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) and the Institute of Molecular Pathology (IMP).
Kaan Boztug, a world-leading expert in primary immunodeficiency research, also leads the Ludwig Boltzmann Institute for Rare and Undiagnosed Diseases (LBI-RUD), founded in 2016, and is strategically located close to the medical campus of AKH and St. Anna Children’s Hospital. The institute is deeply integrated with its renowned partner institutions, namely the CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, the Medical University of Vienna, and the St. Anna Children’s Cancer Research Institute (CCRI), and therefore, perfectly situated to benefit from strong translational research long established by leading-edge research groups.
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Applicants should please submit: A cover letter (describing your career goals and explaining why you are the ideal candidate for this position), a curriculum vitae and names and contact details of 2-3 referees, in a single PDF file to: applicationccri [dot] at
Application deadline: 1st February 2021. Applications will be considered until the position is filled, though.